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RNA Purifications kit

Total RNA Purification Kits

The purification strategies of our Whole RNA Purification Kits vary from natural extraction reagents all the way in which to spin columns and magnetic beads.

Beginning with prime quality, pure, and intact complete RNA is important to many experiments (e.g. RT-PCR, qRT-PCR, array evaluation, Northern blots, nuclease safety assays, and RNA-sequencing). The desk beneath will make it easier to select the precise Invitrogen product to purify complete RNA out of your particular pattern kind and pattern dimension.

Thermo Scientific GeneJET RNA Purification Package is an easy and environment friendly system for purification of complete RNA from mammalian cultured cells, tissue, human blood cells, micro organism, and yeast.

The package makes use of a silica-based membrane know-how within the type of a handy spin column, eliminating the necessity for tedious cesium chloride gradients, alcohol precipitation, or poisonous phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides could be remoted with the GeneJET RNA Purification Package in 15 minutes after the lysis step. The high-quality purified RNA can be utilized in a variety of downstream functions, together with RT-PCR, RT-qPCR, Northern blotting, and different RNA-based analyses.

Highlights

• Common – can be utilized for each cell and tissue samples from a variety of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and micro organism
• Environment friendly – excessive yields of complete RNA.
• Quick – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Handy – spin columns are capped and assembled with assortment tubes

Purposes

• Quick extraction of excessive purity RNA appropriate for all typical molecular biology procedures, together with:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease safety assayIncludes Proteinase Okay Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Assortment Tubes) Assortment Tubes (2 ml) Assortment Tubes (1.5 ml) Detailed Protocol.

Total RNA Purification Kits
Whole RNA Purification Kits

Basic description

Sigma′s GenElute Whole RNA Purification Package gives a speedy technique for the isolation and purification of complete RNA from cultured animal cells, tissue samples, blood, plasma, serum, micro organism, yeast, fungi, vegetation and viruses. The package purifies all sizes of RNA, from giant mRNA and ribosomal RNA right down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from different mobile parts comparable to proteins, with out using phenol or chloroform. The purified RNA is of the very best integrity, and can be utilized in numerous downstream functions together with actual time PCR, reverse transcription PCR, Northern blotting, RNase safety and primer extension, and expression array assays.

  • Isolate true complete RNA together with siRNA and microRNA
  • Quickly take away contaminating genomic DNA with out using enzymes
  • No phenol or chloroform extractions
  • Purify high-quality RNA in 20 minutes
  • Extract RNA from as little as a single cell
  • Isolate from all kinds of specimens

Utility

GenElute Whole RNA Purification Package is used for the extraction of complete RNA:

  • From complete blood samples within the detection of Hepatitis C Virus utilizing a reverse transcriptase-polymerase spiral response (RT-PSR) system.
  • From an in a single day tradition of bacterial strains in TSB medium in figuring out the results of curcumin and silymarin on virulence and carbapenemase genes amongst multidrug-resistant Escherichia coli scientific isolates.
RNA purification
RNA purification

 

GenElute Whole RNA Purification Package has been used to:

  • Quantitative, real-time RT-PCR for each giant mRNA and small RNA together with miRNA
  • RT-PCR for each giant mRNA and small RNA together with miRNA
  • Expression profiling
  • Subsequent Era Sequencing for RNA and miRNA
  • miRNA from plasma for discovery
  • microRNA cloning and amplification
  • PCR-based virus detection
  • PCR-based viable micro organism detection
  • Northern blotting
  • RNase safety
  • Primer extension
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR
  • Isolate complete RNA of rat kidney tissues

MagaBio plus Total RNA Purification Kit

TRI-C53S1C 32T
EUR 831.6

MagaBio plus Total RNA Purification Kit

TRI-C53S1E 32T
EUR 831.6

MagaBio plus Total RNA Purification Kit

TRI-C53S1S 32T
EUR 1073.52

Biospin Blood Total RNA Purification Kit

TRI-C114M1 100T
EUR 579.6

Biospin Blood Total RNA Purification Kit

TRI-C114S1 50T
EUR 315

HiPurA™ 96 Total RNA Purification Kit

MB612-1X96PR 1 unit
EUR 220.5
Description: HiPurA™ 96 Total RNA Purification Kit

MagaBio plus Total RNA Purification Kit II

TRI-C69L1E 96 T
EUR 1512

MagaBio plus Total RNA Purification Kit II

TRI-C69M1B 100T
EUR 1078.56

MagaBio plus Total RNA Purification Kit II

TRI-C69M1E 48 T
EUR 851.76

MagaBio plus Total RNA Purification Kit II

TRI-C69S1B 50T
EUR 579.6

MagaBio plus Total RNA Purification Kit II

TRI-C69S1C 32T
EUR 831.6

MagaBio plus Total RNA Purification Kit II

TRI-C69S1C-C 32T
EUR 504

MagaBio plus Total RNA Purification Kit II

TRI-C69S1E 32T
EUR 831.6

MagaBio plus Total RNA Purification Kit II

TRI-C69S1S 32T
EUR 929.88

MagaBio plus Blood Total RNA Purification Kit

TRI-C113L1E 96T
EUR 1512

MagaBio plus Blood Total RNA Purification Kit

TRI-C113M1 100T
EUR 1253.7

MagaBio plus Blood Total RNA Purification Kit

TRI-C113M1E 48T
EUR 800.1

MagaBio plus Blood Total RNA Purification Kit

TRI-C113S1 50T
EUR 674.1

MagaBio plus Blood Total RNA Purification Kit

TRI-C113S1E 32T
EUR 504

MagaBio plus Blood Total RNA Purification Kit

TRI-C113T1E 16T
EUR 264.6

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