You are currently viewing Total RNA Purification Kits
RNA Purifications kit

Total RNA Purification Kits

The purification strategies of our Whole RNA Purification Kits vary from natural extraction reagents all the way in which to spin columns and magnetic beads.

Beginning with prime quality, pure, and intact complete RNA is important to many experiments (e.g. RT-PCR, qRT-PCR, array evaluation, Northern blots, nuclease safety assays, and RNA-sequencing). The desk beneath will make it easier to select the precise Invitrogen product to purify complete RNA out of your particular pattern kind and pattern dimension.

Thermo Scientific GeneJET RNA Purification Package is an easy and environment friendly system for purification of complete RNA from mammalian cultured cells, tissue, human blood cells, micro organism, and yeast.

The package makes use of a silica-based membrane know-how within the type of a handy spin column, eliminating the necessity for tedious cesium chloride gradients, alcohol precipitation, or poisonous phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides could be remoted with the GeneJET RNA Purification Package in 15 minutes after the lysis step. The high-quality purified RNA can be utilized in a variety of downstream functions, together with RT-PCR, RT-qPCR, Northern blotting, and different RNA-based analyses.


• Common – can be utilized for each cell and tissue samples from a variety of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and micro organism
• Environment friendly – excessive yields of complete RNA.
• Quick – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Handy – spin columns are capped and assembled with assortment tubes


• Quick extraction of excessive purity RNA appropriate for all typical molecular biology procedures, together with:
• Northern blotting
• Nuclease safety assayIncludes Proteinase Okay Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Assortment Tubes) Assortment Tubes (2 ml) Assortment Tubes (1.5 ml) Detailed Protocol.

Total RNA Purification Kits
Whole RNA Purification Kits

Basic description

Sigma′s GenElute Whole RNA Purification Package gives a speedy technique for the isolation and purification of complete RNA from cultured animal cells, tissue samples, blood, plasma, serum, micro organism, yeast, fungi, vegetation and viruses. The package purifies all sizes of RNA, from giant mRNA and ribosomal RNA right down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from different mobile parts comparable to proteins, with out using phenol or chloroform. The purified RNA is of the very best integrity, and can be utilized in numerous downstream functions together with actual time PCR, reverse transcription PCR, Northern blotting, RNase safety and primer extension, and expression array assays.

  • Isolate true complete RNA together with siRNA and microRNA
  • Quickly take away contaminating genomic DNA with out using enzymes
  • No phenol or chloroform extractions
  • Purify high-quality RNA in 20 minutes
  • Extract RNA from as little as a single cell
  • Isolate from all kinds of specimens


GenElute Whole RNA Purification Package is used for the extraction of complete RNA:

  • From complete blood samples within the detection of Hepatitis C Virus utilizing a reverse transcriptase-polymerase spiral response (RT-PSR) system.
  • From an in a single day tradition of bacterial strains in TSB medium in figuring out the results of curcumin and silymarin on virulence and carbapenemase genes amongst multidrug-resistant Escherichia coli scientific isolates.
RNA purification
RNA purification


GenElute Whole RNA Purification Package has been used to:

  • Quantitative, real-time RT-PCR for each giant mRNA and small RNA together with miRNA
  • RT-PCR for each giant mRNA and small RNA together with miRNA
  • Expression profiling
  • Subsequent Era Sequencing for RNA and miRNA
  • miRNA from plasma for discovery
  • microRNA cloning and amplification
  • PCR-based virus detection
  • PCR-based viable micro organism detection
  • Northern blotting
  • RNase safety
  • Primer extension
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR
  • Isolate complete RNA of rat kidney tissues

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 344

Ethanol, DEPC Treated 96% , For RNA Purification

E4500-050 500ml Ask for price

Ethanol, DEPC Treated 70% , For RNA Purification

E4600-050 500ml Ask for price

PCR Purification Kit

  • EUR 326.40
  • EUR 243.60
  • 200 rxns
  • 50 rxns

AfterTri-RNA Purification Mini Kit (200prep)

FAATR-001-1 200 preps
EUR 310.8

After Tri-RNA Purification Mini Kit (50prep)

FAATR-001 50 preps
EUR 175.2

Lysosome Purification Kit

K235-50 each
EUR 601.2

SeraMir Exosome RNA Purification Column kit (20 exoRNA columns)

RA808A-1 20 preps
EUR 344

HCV Mini Purification Kit

K1309-10 each
EUR 802.8

HCV Mini Purification Kit

K1309-20 each
EUR 1096.8

HCV Maxi Purification Kit

K1310-10 each
EUR 1683.6

HCV Maxi Purification Kit

K1310-2 each
EUR 639.6

HCV Maxi Purification Kit

K1310-4 each
EUR 1083.6

Adenovirus Purification Kit

K1459-100 100 Preps
EUR 1244.4

Adenovirus Purification Kit

K1459-20 20 Preps
EUR 727.2

His-tag Purification Kit

abx299036-4U 4 U
EUR 427.2

Sperm DNA Purification Kit

K1465-250 250 Preps
EUR 661.2

Sperm DNA Purification Kit

K1465-50 50 Preps
EUR 381.6

Stool DNA Purification Kit

K1474-250 250 Preps
EUR 721.2

Stool DNA Purification Kit

K1474-50 50 Preps
EUR 417.6

Leave a Reply