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RNA Purifications kit

Total RNA Purification Kits

The purification strategies of our Whole RNA Purification Kits vary from natural extraction reagents all the way in which to spin columns and magnetic beads.

Beginning with prime quality, pure, and intact complete RNA is important to many experiments (e.g. RT-PCR, qRT-PCR, array evaluation, Northern blots, nuclease safety assays, and RNA-sequencing). The desk beneath will make it easier to select the precise Invitrogen product to purify complete RNA out of your particular pattern kind and pattern dimension.

Thermo Scientific GeneJET RNA Purification Package is an easy and environment friendly system for purification of complete RNA from mammalian cultured cells, tissue, human blood cells, micro organism, and yeast.

The package makes use of a silica-based membrane know-how within the type of a handy spin column, eliminating the necessity for tedious cesium chloride gradients, alcohol precipitation, or poisonous phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides could be remoted with the GeneJET RNA Purification Package in 15 minutes after the lysis step. The high-quality purified RNA can be utilized in a variety of downstream functions, together with RT-PCR, RT-qPCR, Northern blotting, and different RNA-based analyses.

Highlights

• Common – can be utilized for each cell and tissue samples from a variety of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and micro organism
• Environment friendly – excessive yields of complete RNA.
• Quick – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Handy – spin columns are capped and assembled with assortment tubes

Purposes

• Quick extraction of excessive purity RNA appropriate for all typical molecular biology procedures, together with:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease safety assayIncludes Proteinase Okay Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Assortment Tubes) Assortment Tubes (2 ml) Assortment Tubes (1.5 ml) Detailed Protocol.

Total RNA Purification Kits
Whole RNA Purification Kits

Basic description

Sigma′s GenElute™ Whole RNA Purification Package gives a speedy technique for the isolation and purification of complete RNA from cultured animal cells, tissue samples, blood, plasma, serum, micro organism, yeast, fungi, vegetation and viruses. The package purifies all sizes of RNA, from giant mRNA and ribosomal RNA right down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from different mobile parts comparable to proteins, with out using phenol or chloroform. The purified RNA is of the very best integrity, and can be utilized in numerous downstream functions together with actual time PCR, reverse transcription PCR, Northern blotting, RNase safety and primer extension, and expression array assays.

  • Isolate true complete RNA together with siRNA and microRNA
  • Quickly take away contaminating genomic DNA with out using enzymes
  • No phenol or chloroform extractions
  • Purify high-quality RNA in 20 minutes
  • Extract RNA from as little as a single cell
  • Isolate from all kinds of specimens

Utility

GenElute Whole RNA Purification Package is used for the extraction of complete RNA:

  • From complete blood samples within the detection of Hepatitis C Virus utilizing a reverse transcriptase-polymerase spiral response (RT-PSR) system.
  • From an in a single day tradition of bacterial strains in TSB medium in figuring out the results of curcumin and silymarin on virulence and carbapenemase genes amongst multidrug-resistant Escherichia coli scientific isolates.
RNA purification
RNA purification

 

GenElute Whole RNA Purification Package has been used to:

  • Quantitative, real-time RT-PCR for each giant mRNA and small RNA together with miRNA
  • RT-PCR for each giant mRNA and small RNA together with miRNA
  • Expression profiling
  • Subsequent Era Sequencing for RNA and miRNA
  • miRNA from plasma for discovery
  • microRNA cloning and amplification
  • PCR-based virus detection
  • PCR-based viable micro organism detection
  • Northern blotting
  • RNase safety
  • Primer extension
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR
  • Isolate complete RNA of rat kidney tissues

PureXtract RNA Purification Kit
R6111-200 200 prep
EUR 1048.8

Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL 96 assays
EUR 686.4

RNA isolater Total RNA Extraction Reagent
R401-01 100 ml
EUR 159.6

Navy RNA Lysis Kit 50
NAVYE1-RNA 50pack
EUR 237.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250
NAVYE5-RNA 250pack
EUR 781.2
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Green RNA Lysis Kit 50
GREENE1-RNA 50pack
EUR 216
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250
GREENE5-RNA 250pack
EUR 685.2
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Pink RNA Lysis Kit 50
PINKE1-RNA 50pack
EUR 213.6
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250
PINKE5-RNA 250pack
EUR 673.2
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50
REDE1-RNA 50pack
EUR 230.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250
REDE5-RNA 250pack
EUR 746.4
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50
YELLOWE1-RNA 50pack
EUR 230.4
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250
YELLOWE5-RNA 250pack
EUR 748.8
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Total RNA Isolation Kit
P-9100 50 samples
EUR 416.7

RNA/DNA Purification Kit (Magnetic Bead)
DA0623 32 test/kit Ask for price

AfterTri-RNA Purification Mini Kit (200prep)
FAATR-001-1 200 preps
EUR 310.8

ExoQuick Exosome RNA Column Purification kit
EQ808A-1 20 preps
EUR 482.4

5E Navy RNA Lysis Kit 100
NAVY5E100-RNA 100pack
EUR 543.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20
NAVY5E20-RNA 20pack
EUR 183.6
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50
NAVYR1-RNA 50pack
EUR 242.4
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

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