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RNA Purifications kit

Total RNA Purification Kits

The purification strategies of our Whole RNA Purification Kits vary from natural extraction reagents all the way in which to spin columns and magnetic beads.

Beginning with prime quality, pure, and intact complete RNA is important to many experiments (e.g. RT-PCR, qRT-PCR, array evaluation, Northern blots, nuclease safety assays, and RNA-sequencing). The desk beneath will make it easier to select the precise Invitrogen product to purify complete RNA out of your particular pattern kind and pattern dimension.

Thermo Scientific GeneJET RNA Purification Package is an easy and environment friendly system for purification of complete RNA from mammalian cultured cells, tissue, human blood cells, micro organism, and yeast.

The package makes use of a silica-based membrane know-how within the type of a handy spin column, eliminating the necessity for tedious cesium chloride gradients, alcohol precipitation, or poisonous phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides could be remoted with the GeneJET RNA Purification Package in 15 minutes after the lysis step. The high-quality purified RNA can be utilized in a variety of downstream functions, together with RT-PCR, RT-qPCR, Northern blotting, and different RNA-based analyses.

Highlights

• Common – can be utilized for each cell and tissue samples from a variety of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and micro organism
• Environment friendly – excessive yields of complete RNA.
• Quick – 15 minutes after lysis step
• Pure – an A260/280 ratio > 1.9
• Handy – spin columns are capped and assembled with assortment tubes

Purposes

• Quick extraction of excessive purity RNA appropriate for all typical molecular biology procedures, together with:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease safety assayIncludes Proteinase Okay Lysis Buffer Wash Buffer 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with Assortment Tubes) Assortment Tubes (2 ml) Assortment Tubes (1.5 ml) Detailed Protocol.

Total RNA Purification Kits
Whole RNA Purification Kits

Basic description

Sigma′s GenElute Whole RNA Purification Package gives a speedy technique for the isolation and purification of complete RNA from cultured animal cells, tissue samples, blood, plasma, serum, micro organism, yeast, fungi, vegetation and viruses. The package purifies all sizes of RNA, from giant mRNA and ribosomal RNA right down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from different mobile parts comparable to proteins, with out using phenol or chloroform. The purified RNA is of the very best integrity, and can be utilized in numerous downstream functions together with actual time PCR, reverse transcription PCR, Northern blotting, RNase safety and primer extension, and expression array assays.

  • Isolate true complete RNA together with siRNA and microRNA
  • Quickly take away contaminating genomic DNA with out using enzymes
  • No phenol or chloroform extractions
  • Purify high-quality RNA in 20 minutes
  • Extract RNA from as little as a single cell
  • Isolate from all kinds of specimens

Utility

GenElute Whole RNA Purification Package is used for the extraction of complete RNA:

  • From complete blood samples within the detection of Hepatitis C Virus utilizing a reverse transcriptase-polymerase spiral response (RT-PSR) system.
  • From an in a single day tradition of bacterial strains in TSB medium in figuring out the results of curcumin and silymarin on virulence and carbapenemase genes amongst multidrug-resistant Escherichia coli scientific isolates.
RNA purification
RNA purification

 

GenElute Whole RNA Purification Package has been used to:

  • Quantitative, real-time RT-PCR for each giant mRNA and small RNA together with miRNA
  • RT-PCR for each giant mRNA and small RNA together with miRNA
  • Expression profiling
  • Subsequent Era Sequencing for RNA and miRNA
  • miRNA from plasma for discovery
  • microRNA cloning and amplification
  • PCR-based virus detection
  • PCR-based viable micro organism detection
  • Northern blotting
  • RNase safety
  • Primer extension
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR
  • Isolate complete RNA of rat kidney tissues

RNA Purification Kit

MB1006-250preps 250 preps
EUR 397

RNA Purification Kit

MB1006-50preps 50 preps
EUR 166

PureXtract RNA Purification Kit

R6111-050 50 prep
EUR 280

PureXtract RNA Purification Kit

R6111-200 200 prep
EUR 874

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL 96 assays
EUR 572

RNA isolater Total RNA Extraction Reagent

R401-01 100 ml
EUR 133

Green RNA Lysis Kit 50

GREENE1-RNA 50pack
EUR 180
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250

GREENE5-RNA 250pack
EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Navy RNA Lysis Kit 50

NAVYE1-RNA 50pack
EUR 198
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250

NAVYE5-RNA 250pack
EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Pink RNA Lysis Kit 50

PINKE1-RNA 50pack
EUR 178
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250

PINKE5-RNA 250pack
EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50

REDE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250

REDE5-RNA 250pack
EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50

YELLOWE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250

YELLOWE5-RNA 250pack
EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

AfterTri-RNA Purification Mini Kit (200prep)

FAATR-001-1 200 preps
EUR 259

ExoQuick Exosome RNA Column Purification kit

EQ808A-1 20 preps
EUR 402

Total RNA Extraction Kit

K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA Isolation Kit

P-9100 50 samples
EUR 347.25
Description: The best epigenetics products

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