The purification strategies of our Whole RNA Purification Kits vary from natural extraction reagents all the way in which to spin columns and magnetic beads.
Beginning with prime quality, pure, and intact complete RNA is important to many experiments (e.g. RT-PCR, qRT-PCR, array evaluation, Northern blots, nuclease safety assays, and RNA-sequencing). The desk beneath will make it easier to select the precise Invitrogen product to purify complete RNA out of your particular pattern kind and pattern dimension.
Thermo Scientific GeneJET RNA Purification Package is an easy and environment friendly system for purification of complete RNA from mammalian cultured cells, tissue, human blood cells, micro organism, and yeast.
The package makes use of a silica-based membrane know-how within the type of a handy spin column, eliminating the necessity for tedious cesium chloride gradients, alcohol precipitation, or poisonous phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides could be remoted with the GeneJET RNA Purification Package in 15 minutes after the lysis step. The high-quality purified RNA can be utilized in a variety of downstream functions, together with RT-PCR, RT-qPCR, Northern blotting, and different RNA-based analyses.
Highlights
• Common – can be utilized for each cell and tissue samples from a variety of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and micro organism • Environment friendly – excessive yields of complete RNA. • Quick – 15 minutes after lysis step • Pure – an A260/280 ratio > 1.9 • Handy – spin columns are capped and assembled with assortment tubes
Sigma′s GenElute™ Whole RNA Purification Package gives a speedy technique for the isolation and purification of complete RNA from cultured animal cells, tissue samples, blood, plasma, serum, micro organism, yeast, fungi, vegetation and viruses. The package purifies all sizes of RNA, from giant mRNA and ribosomal RNA right down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from different mobile parts comparable to proteins, with out using phenol or chloroform. The purified RNA is of the very best integrity, and can be utilized in numerous downstream functions together with actual time PCR, reverse transcription PCR, Northern blotting, RNase safety and primer extension, and expression array assays.
Isolate true complete RNA together with siRNA and microRNA
Quickly take away contaminating genomic DNA with out using enzymes
No phenol or chloroform extractions
Purify high-quality RNA in 20 minutes
Extract RNA from as little as a single cell
Isolate from all kinds of specimens
Utility
GenElute Whole RNA Purification Package is used for the extraction of complete RNA:
From complete blood samples within the detection of Hepatitis C Virus utilizing a reverse transcriptase-polymerase spiral response (RT-PSR) system.
From an in a single day tradition of bacterial strains in TSB medium in figuring out the results of curcumin and silymarin on virulence and carbapenemase genes amongst multidrug-resistant Escherichia coli scientific isolates.
RNA purification
GenElute Whole RNA Purification Package has been used to:
Quantitative, real-time RT-PCR for each giant mRNA and small RNA together with miRNA
RT-PCR for each giant mRNA and small RNA together with miRNA
