Essentially the most well-liked and fixed embedding medium for cryotomy
Tissue-Tek O.C.T. Compound is used to shortly embed up to date tissue specimens for frozen sections using a cryostat. Tissue-Tek O.C.T. Compound is a formulation of clear, water-soluble glycols and resins, providing a steady matrix to encapsulate tissue specimens and bond to the Specimen Holder for fixed sectioning in a cryostat working temperature of -10°C and beneath.
Undesirable background staining is eradicated as no residue is left on the slides all through staining.
O.C.T stands for optimum chopping temperature. The extreme viscosity of Tissue-Tek O.C.T Compound ends in fast freezing for optimum half top quality.
The OCT Compound is purchased in 4 oz (118 mL) squeeze bottles; 12/case.
What’s exactly in TissueTek OCT compound and what’s its affect on enzyme train of tissues?
Hey all, I am working with cryo pores and pores and skin samples and I am trying to do in situ zymography (visualize enzyme acitivity with substrate which turns into fluorescent). The pores and pores and skin samples are prepared with TissueTek, then snap-freezed in liquid nitrogen after which decrease using a cryostat. Nonetheless, I cannot uncover what’s exactly in TissueTek, as a result of it may very well be important rationalization for the affect on enzyme train (pH?). Does anyone know if embedding tissues with TissueTek might affect enzyme train in tissues?
Choices and benefits of the Tissue-Tek OCT embedding compound are:
Water soluable mixture of glycols and resins give a gradual embedding matrix
Superior half top quality at cryostat temperatures from -10°C to -40°C
Extraordinarily soluable in water, alcohol and formalin
Good sectioning properties with disposable cryostat blades and steady knives
Would not go away residues on slides all through staining procedures, eliminating undesirable background staining
Tissue, mounted in OCT compound shall be frozen between -10°C and -80°C
A wonderful mounting medium for cryo-SEM, cryo-FIB/SEM and totally different chilly phases
Specs for this merchandise
Mannequin Establish
EMS
Merchandise Weight
113 grams
Model Amount
62550-01
Number of Devices
1
Half Amount
62550-01
Tissue Clear Containing 5L
Product particulars
Is Discontinued By Producer : No
Package deal deal Dimensions : 19.three x 5.three x 4 cm; 113.4 Grams
Date First Accessible : 13 January 2020
Producer : Electron Microscopy Sciences
ASIN : B00Okay33LEBS
Merchandise model amount : 62550-01
Producer : Electron Microscopy Sciences
Merchandise Weight : 113 g
Internet Quantity : 1 Reply
The Tissue-Tek optimum chopping temperature (O.C.T.) decision consists of water-soluble glycols and resins that give a specimen matrix for cryostat sectioning at temperatures of –10°C (14°F) and beneath. This product would not go away any residue on slides all through and after the staining course. It is packaged and purchased individually in a 118mL (4oz.) bottle.
