Key choices and particulars

 

• Sensitivity: 10 pg/ml
• Fluctuate: 78 pg/ml – 5000 pg/ml
• Sample kind: Cell custom supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
• Detection method: Colorimetric
• Assay kind: Sandwich (quantitative)
• Reacts with: Human

 

• Product determine

  Human BAFF-R ELISA Gear

• Detection method

  Colorimetric

• Precision

  Intra-assay

  Sample	n	Indicate	SD	CV%
  1	16	147pg/ml	10.29	= 7%
  2	16	683pg/ml	49.85	= 7.3%
  3	16	2750pg/ml	206.25	= 7.5%

  Inter-assay

  Sample	n	Indicate	SD	CV%
  1	24	156pg/ml	13.88	= 8.9%
  2	24	700pg/ml	62.3	= 8.9%
  3	24	2531pg/ml	227.05	= 8.9%

  

• Sample kind

  Cell custom supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma

• Assay kind

  Sandwich (quantitative)

• Sensitivity

  < 10 pg/ml

• Fluctuate

  78 pg/ml – 5000 pg/ml

• Assay time

  3h 30m

• Assay size

  Numerous steps commonplace assay

• Species reactivity

  Reacts with: Human

• Product overview

  The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) tools (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom supernatants, cell lysates, serum and plasma (heparin, EDTA).

  The ELISA tools is based on commonplace sandwich enzyme-linked immune-sorbent assay experience. A monoclonal antibody from mouse explicit for BAFF-R has been pre-coated onto 96-well plates. Necessities (Expression system for regular: NSO; Immunogen sequence: S7-A71) and check out samples are added to the wells, a biotinylated detection polyclonal antibody from goat explicit for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Sophisticated is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to produce a blue shade product that turned yellow after together with acidic stop reply. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.

• Notes

  BAFF-R (additionally known as Tumor necrosis situation receptor superfamily member 13C or B-cell-activating situation receptor)  is a protein that in folks is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating situation (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a sort III transmembrane protein containing a single extracellular phenylalanine-rich space. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

• Platform

  Pre-coated microplate (12 x Eight correctly strips)

Histone H3 Peptide (residues 15-39)
R-1005	EpiGentek	EUR 303.60 EUR 94.60 EUR 165.00	200 µl 100 ul 200 ul

Histone H3 Peptide (residues 21-44), Biotinylated
R-1006	EpiGentek	EUR 362.76 EUR 119.90 EUR 205.70	200 µl 100 ul 200 ul

Histone H4 Peptide (residues 1-21), Biotinylated
R-1007	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H4ac Peptide, Biotinylated
R-1008	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H3K9ac Peptide
R-1009	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H3K9ac Peptide, Biotinylated
R-1010	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H3K14ac Peptide
R-1011	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H3K14ac Peptide, Biotinylated
R-1012	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone Acetyl H4K5ac Peptide
R-1014	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H4K8ac Peptide
R-1015	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H4K12ac Peptide
R-1016	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone Acetyl H4K16ac Peptide
R-1017	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone H3K4me1 Monomethyl Peptide
R-1018	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K4me1 Monomethyl Peptide, Biotinylated
R-1019	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K4me2 Dimethyl Peptide
R-1020	EpiGentek	EUR 267.06 EUR 78.10 EUR 140.80	200 µl 100 ul 200 ul

Histone H3K4me2 Dimethyl Peptide, Biotinylated
R-1021	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K4me3 Trimethyl Peptide
R-1022	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K4me3 Trimethyl Peptide, Biotinylated
R-1023	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K9me1 Monomethyl Peptide
R-1024	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K9me1 Monomethyl Peptide, Biotinylated
R-1025	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K9me2 Dimethyl Peptide
R-1026	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K9me2 Dimethyl Peptide, Biotinylated
R-1027	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K9me3 Trimethyl Peptide
R-1028	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K9me3 Trimethyl Peptide, Biotinylated
R-1029	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul

Histone H3K27me1 Monomethyl Peptide
R-1030	EpiGentek	EUR 341.88 EUR 106.70 EUR 191.40	200 µl 100 ul 200 ul

Histone H3K27me1 Monomethyl Peptide, Biotinylated
R-1031	EpiGentek	EUR 361.02 EUR 119.90 EUR 204.60	200 µl 100 ul 200 ul