Key choices and particulars
- Sensitivity: 10 pg/ml
- Fluctuate: 78 pg/ml – 5000 pg/ml
- Sample kind: Cell custom supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay kind: Sandwich (quantitative)
- Reacts with: Human
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Product determine
Human BAFF-R ELISA Gear -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Indicate SD CV% 1 16 147pg/ml 10.29 = 7% 2 16 683pg/ml 49.85 = 7.3% 3 16 2750pg/ml 206.25 = 7.5% Inter-assay Sample n Indicate SD CV% 1 24 156pg/ml 13.88 = 8.9% 2 24 700pg/ml 62.3 = 8.9% 3 24 2531pg/ml 227.05 = 8.9% bio human baff r -
Sample kind
Cell custom supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay kind
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Fluctuate
78 pg/ml – 5000 pg/ml -
Assay time
3h 30m -
Assay size
Numerous steps commonplace assay -
Species reactivity
Reacts with: Human -
Product overview
The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) tools (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA tools is based on commonplace sandwich enzyme-linked immune-sorbent assay experience. A monoclonal antibody from mouse explicit for BAFF-R has been pre-coated onto 96-well plates. Necessities (Expression system for regular: NSO; Immunogen sequence: S7-A71) and check out samples are added to the wells, a biotinylated detection polyclonal antibody from goat explicit for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Sophisticated is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to produce a blue shade product that turned yellow after together with acidic stop reply. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.
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Notes
BAFF-R (additionally known as Tumor necrosis situation receptor superfamily member 13C or B-cell-activating situation receptor) is a protein that in folks is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating situation (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a sort III transmembrane protein containing a single extracellular phenylalanine-rich space. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
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Platform
Pre-coated microplate (12 x Eight correctly strips)
Histone H3 Peptide (residues 15-39) |
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R-1005 | EpiGentek |
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Histone H3 Peptide (residues 21-44), Biotinylated |
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R-1006 | EpiGentek |
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Histone H4 Peptide (residues 1-21), Biotinylated |
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R-1007 | EpiGentek |
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Histone Acetyl H4ac Peptide, Biotinylated |
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R-1008 | EpiGentek |
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Histone Acetyl H3K9ac Peptide |
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R-1009 | EpiGentek |
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Histone Acetyl H3K9ac Peptide, Biotinylated |
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R-1010 | EpiGentek |
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Histone Acetyl H3K14ac Peptide |
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R-1011 | EpiGentek |
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Histone Acetyl H3K14ac Peptide, Biotinylated |
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R-1012 | EpiGentek |
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Histone Acetyl H4K5ac Peptide |
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R-1014 | EpiGentek |
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Histone Acetyl H4K8ac Peptide |
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R-1015 | EpiGentek |
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Histone Acetyl H4K12ac Peptide |
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R-1016 | EpiGentek |
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Histone Acetyl H4K16ac Peptide |
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R-1017 | EpiGentek |
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Histone H3K4me1 Monomethyl Peptide |
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R-1018 | EpiGentek |
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Histone H3K4me1 Monomethyl Peptide, Biotinylated |
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R-1019 | EpiGentek |
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Histone H3K4me2 Dimethyl Peptide |
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R-1020 | EpiGentek |
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Histone H3K4me2 Dimethyl Peptide, Biotinylated |
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R-1021 | EpiGentek |
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Histone H3K4me3 Trimethyl Peptide |
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R-1022 | EpiGentek |
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Histone H3K4me3 Trimethyl Peptide, Biotinylated |
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R-1023 | EpiGentek |
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Histone H3K9me1 Monomethyl Peptide |
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R-1024 | EpiGentek |
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Histone H3K9me1 Monomethyl Peptide, Biotinylated |
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R-1025 | EpiGentek |
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Histone H3K9me2 Dimethyl Peptide |
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R-1026 | EpiGentek |
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Histone H3K9me2 Dimethyl Peptide, Biotinylated |
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R-1027 | EpiGentek |
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Histone H3K9me3 Trimethyl Peptide |
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R-1028 | EpiGentek |
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Histone H3K9me3 Trimethyl Peptide, Biotinylated |
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R-1029 | EpiGentek |
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Histone H3K27me1 Monomethyl Peptide |
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R-1030 | EpiGentek |
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Histone H3K27me1 Monomethyl Peptide, Biotinylated |
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R-1031 | EpiGentek |
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