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Proteinase K, recombinant

Proteinase K, recombinant

Proteinase Okay – one of many typically used enzymes in molecular biology.

Wonderful different for protein digestion in various capabilities.

  • BLIRT – skilled in Proteinase Okay manufacturing for over 15 years.
  • Licensed manufacturing course of ISO 13485 ensuing throughout the highest prime quality product with a extremely low batch-to-batch variability.
  • Large parts, the chance of white labeling, and portioning service – liquid form throughout the amount on the client’s request (as a lot as a variety of million samples month-to-month).

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even throughout the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Proteinase Okay (Lyophilized)

For Fundamental Digestion of Protein in Natural Samples

  • Energetic over a pH range of 4.3–12.0, in 0.5% SDS or 1% Triton X-100
  • Purified to remove RNase and DNase actions

Safe at Room Temperature and Easy to Use

  • Energetic over pH range 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% train at temperatures as a lot as 60°C
  • No resuspension or thawing sooner than use
  • Cat.# MC5005, MC5008 supplied at a spotlight of 20mg/ml



Highlights

• Ready-to-use reply
• Energetic in quite a lot of response circumstances

Features

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Remember

• The actually helpful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The train of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to 4 M urea
• Ca2+ protects Proteinase Okay in the direction of autolysis, will improve the thermal stability, and has a regulatory carry out for the substrate binding website online of Proteinase Okay
• Safe over a big pH range: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum train at 50 to55°C
• Speedy denaturation of enzyme occurs at temperatures above 65°C.

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad-spectrum endopeptidase with a extremely extreme specific train.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the perfect prime quality product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is vigorous beneath quite a lot of response circumstances, along with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively using for the digestion of proteins, along with DNases and RNases, all through nucleic acid preparations with out compromising the integrity of isolated DNA or RNA.

Proteinase K, recombinant
Proteinase Okay, recombinant

Choices

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Extreme train and distinctive purity.
  • Energetic at extreme temperatures (as a lot as 56 °C) and denaturing circumstances (e.g. throughout the presence of urea and/or SDS), which makes it perfect for digesting proteins in a variety of capabilities.
  • Safe over a big pH range: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Features:

  • Extraction of DNA and RNA from completely completely different starting provides.
  • Purification of purpose supplies from contaminating proteins.
  • Elimination of DNases and RNases all through nucleic acids isolation.

Proteinase K

AR0056 5mL
EUR 121.2

Proteinase K

G029 1.0 ml
EUR 170.4

Proteinase K

GE0990-100MG 100 mg
EUR 112.8

Proteinase K

GE0990-1G 1 g
EUR 495.6

Proteinase K

GE0990-250MG 250 mg
EUR 184.8

Proteinase K

GE0990-25MG 25 mg
EUR 69.6

Proteinase K

GE0990-500MG 500 mg
EUR 298.8

Proteinase K

K1037-1 1 ml
EUR 80.4
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K

K1037-5 5 ml
EUR 129.6
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K

P2180-010 100mg
EUR 218.4

Proteinase K

P2180-050 500mg
EUR 448.8

Proteinase K

P2180-100 1g
EUR 627.6

Proteinase K

Z5050002 100 mg
EUR 205.2
Description: No genetic downstream application can do without the good old Proteinase K.

Proteinase K

M-CO-404 100 mg
EUR 376
Description: Proteinase K

Proteinase K Antibody

6210-100
EUR 352.8

Proteinase K Antibody

6210-30T
EUR 175.2

PROTEINASE K SOLUTION

501-PK 5 ml
EUR 182.4
Description: Genomic PCR Grade Proteinase K Solution

Proteinase K protein

30R-2901 500 mg
EUR 510
Description: Purified recombinant Human Proteinase K protein

Proteinase K Solution

BIO-37084 5ml @ 20mg/ml Ask for price

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