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Proteinase K, recombinant

Proteinase K, recombinant

Proteinase Okay – one of many typically used enzymes in molecular biology.

Wonderful different for protein digestion in various capabilities.

  • BLIRT – skilled in Proteinase Okay manufacturing for over 15 years.
  • Licensed manufacturing course of ISO 13485 ensuing throughout the highest prime quality product with a extremely low batch-to-batch variability.
  • Large parts, the chance of white labeling, and portioning service – liquid form throughout the amount on the client’s request (as a lot as a variety of million samples month-to-month).

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even throughout the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Proteinase Okay (Lyophilized)

For Fundamental Digestion of Protein in Natural Samples

  • Energetic over a pH range of 4.3–12.0, in 0.5% SDS or 1% Triton X-100
  • Purified to remove RNase and DNase actions

Safe at Room Temperature and Easy to Use

  • Energetic over pH range 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% train at temperatures as a lot as 60°C
  • No resuspension or thawing sooner than use
  • Cat.# MC5005, MC5008 supplied at a spotlight of 20mg/ml



Highlights

• Ready-to-use reply
• Energetic in quite a lot of response circumstances

Features

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Remember

• The actually helpful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The train of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to 4 M urea
• Ca2+ protects Proteinase Okay in the direction of autolysis, will improve the thermal stability, and has a regulatory carry out for the substrate binding website online of Proteinase Okay
• Safe over a big pH range: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum train at 50 to55°C
• Speedy denaturation of enzyme occurs at temperatures above 65°C.

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad-spectrum endopeptidase with a extremely extreme specific train.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the perfect prime quality product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is vigorous beneath quite a lot of response circumstances, along with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively using for the digestion of proteins, along with DNases and RNases, all through nucleic acid preparations with out compromising the integrity of isolated DNA or RNA.

Proteinase K, recombinant
Proteinase Okay, recombinant

Choices

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Extreme train and distinctive purity.
  • Energetic at extreme temperatures (as a lot as 56 °C) and denaturing circumstances (e.g. throughout the presence of urea and/or SDS), which makes it perfect for digesting proteins in a variety of capabilities.
  • Safe over a big pH range: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Features:

  • Extraction of DNA and RNA from completely completely different starting provides.
  • Purification of purpose supplies from contaminating proteins.
  • Elimination of DNases and RNases all through nucleic acids isolation.

Proteinase K

BIO-37039 1000mg Ask for price

Proteinase K

G029 1.0 ml
EUR 142

Proteinase K

GE0990-100MG 100 mg
EUR 94

Proteinase K

GE0990-1G 1 g
EUR 413

Proteinase K

GE0990-250MG 250 mg
EUR 154

Proteinase K

GE0990-25MG 25 mg
EUR 58

Proteinase K

GE0990-500MG 500 mg
EUR 249

Proteinase K

abx082041-100mg 100 mg
EUR 258
  • Shipped within 5-10 working days.

Proteinase K

abx098140-1ml 1 ml
EUR 189
  • Shipped within 5-10 working days.

Proteinase K

K1037-1 1 ml
EUR 67
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K

K1037-5 5 ml
EUR 108
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K

P2180-010 100mg
EUR 182

Proteinase K

P2180-050 500mg
EUR 374

Proteinase K

P2180-100 1g
EUR 523

Proteinase K

Z5050002 100 mg
EUR 171
Description: No genetic downstream application can do without the good old Proteinase K.

Proteinase K Solution

BIO-37084 5ml @ 20mg/ml Ask for price

Proteinase K Solution

BIO-37085 5 x 5ml @ 20mg/ml Ask for price

Proteinase K solution

GZ5904-100ML 100 ml
EUR 907

Proteinase K solution

GZ5904-25ML 25 ml
EUR 349

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