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Direct PCR

Phire and Phusion Kits

What’s Direct PCR?

Direct PCR (dPCR) is a technique of DNA amplification instantly from an animal or plant tissue pattern with out performing DNA isolation and purification steps. This system vastly reduces experimental time, and price in genotyping and high-volume tasks. It additionally supplies a greater choice when confronted with the challenges of amplifying a really small amount of pattern the place the purification step might doubtlessly result in pattern loss.

In some ways, dPCR works like typical PCR. Each strategies rely upon template DNA, primers and a grasp combine or PCR reagents, and each use a thermal cycler for amplification. The first distinction between dPCR and traditional PCR is the tailor-made buffers utilized in dPCR. The modified parts of dPCR allow strong amplification regardless of the presence of PCR inhibitors typically present in crude samples.

ALS SARS-CoV-2 RT-PCR 4G has been developed and produced solely at ALS in Portugal and is designed primarily for export. This new equipment is ready to detect all recognized SARS-CoV-2 variants, together with British, Brazilian and South African variants, with out cross-reacting with different recognized associated viruses or widespread respiratory pathogens. The selection of the genomic areas used as targets and the sequences of the primers and hydrolysis probes have been based mostly on WHO, CDC and DGS suggestions.

The ALS SARS-CoV-2 RT-PCR 4G equipment is an in vitro diagnostic medical machine (CE-IVD) that makes use of real-time PCR nucleic acid amplification expertise for the detection of SARS-CoV-2 in medical samples from the higher respiratory tract of people who might or might not be suspected of getting COVID-19.

The equipment makes use of the RT-PCR approach, combining amplification of sure areas of the viral genome with its detection by particular hydrolysis probes. The SARS-CoV-2 genome goal areas are extremely conserved and correspond to 2 particular genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The equipment may detect the gene that codifies the structural protein of the envelope (gene E) particular to the Sarbecovirus subgenus.

Direct PCR Reagent
Direct PCR Reagent

Benefits

  • Speedy amplification: The amplification price of the polymerase is 6kb/min. 1kb fragment might be amplified inside 25min.
  • Lengthy fragment amplification: For plasmid, λ DNA and different simple templates, the polymerase can successfully amplify > 20kb. For genome, the polymerase can successfully amplify > 8kb. And for cDNA, the polymerase can successfully amplify > 8kb.
  • Excessive specificity: With scorching begin expertise, the polymerase is 100% inactive beneath 50°C, and might solely be restored by heating at 95°C for 5min.
  • Excessive tolerance to impurities: Samples of entire blood, serum, cultured cells and urine might be instantly amplified with out prior DNA purification.

Unit Definition

One unit is outlined as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.

Description Code Specification Class Know-how
ALS SARS-CoV-2 RT-PCR Check for surfaces ALS-B01-Adv RUO Meals /Water Virology Detection Actual-Time PCR
ALS SARS-CoV-2 RT-PCR Check for Wastewater ALS-B02-Adv RUO Meals /Water Virology Detection Actual-Time PCR

 

Direct PCR Complete Kit

S700 100 rcs
EUR 159.6

Direct PCR Complete Kit

S700-2 5x100 rcs
EUR 628.8

Direct Blood PCR Kit

MBPCR171-100R 1 unit
EUR 267.52
Description: Direct Blood PCR Kit

Direct Blood PCR Kit

MBPCR171-10R 1 unit
EUR 71.73
Description: Direct Blood PCR Kit

Blood Direct PCR Kit V2

PD103-01 50 rxn
EUR 51.41

Blood Direct PCR Kit V2

PD103-02 200 rxn
EUR 188.5

Mouse tissue direct PCR kit

EP033 50T
EUR 125

Mouse Tissue Direct PCR Kit

RK20736 100RXN
EUR 150

amfiSure Direct PCR Lysis Buffer

D0300-010 100ml
EUR 165.6

Mouse Direct PCR Kit (For Genotyping)

B40013 200 ractions
EUR 250.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B40015 500 ractions
EUR 490.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45012 1ml*5 (500 ractions)
EUR 336
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45013 1ml*20 (2000 ractions)
EUR 1038
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02100 100 RXNS Ask for price

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02500 500 RXNS Ask for price

FastAmp™ Plant Direct PCR Kit - 1,250 Reactions

4615 1/EA
EUR 495
Description: Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps. The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity and yield. The FastAmpTM Plant Direct PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis.Highlights:Direct PCR- no need to purify DNASpecially engineered Taq DNA polymerase with highest sensitivity and specificityExtremely short PCR protocol timesMaster mix format with premixed gel loading dye to reduce cross-contamination and sample handlingerrors5x magic enhancer for high GC containing DNA amplificationProduct Includes:FastAmp™ plant direct PCR master mix (2x)Dilution bufferControl primer mix (25 µM each)5x magic enhancerNuclease- free water

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02100 100 RXNS Ask for price

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02500 500 RXNS Ask for price

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-540 20 rcs x 50 µl
EUR 196.8

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-542 100 rcs x 50 µl
EUR 658.8

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-544 1000 rcs x 50 µl
EUR 5026.8

FastAmp® Plant Direct PCR & Genotyping Solution - 20mL

4611 20mL
EUR 195
Description: FastAmp® Plant Direct PCR/Genotyping Solution for is designed for rapid cell lysis and DNA separation simultaneously without the need of DNA extraction steps . It contains very low concentration of less toxic chemicals for direct DNA amplification of genomic DNA from various plant tissues. No heat treatment and no termination buffer is is required, saving significant time. FastAmp® Plant Direct PCR/Genotyping Solution has been optimized with FastAmp® Plant Direct PCR kit and FastAmp® Plant Tissue/Seed Genotyping PCR kit.

Direct PCRKits for Blood

FYT302-100P 100 Preps Ask for price

Direct PCRKits for Plant

FYT303-100P 100 Preps Ask for price

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-100P 100 Preps Ask for price

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-50P 50 Preps Ask for price

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-100P 100 Preps Ask for price

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-50P 50 Preps; Ask for price
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