Lipid Peroxidation (MDA + HNE) Assay Package

Lipid peroxidation is a well known instance of oxidative injury in cell membranes, lipoproteins, and different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl cholesterol can happen in several reactions. They are often triggered by i) free radical species comparable to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated discount of hydrogen peroxide or ii) non-radical species comparable to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are essential poisonous byproducts of lipid peroxidation. So, the measurement of the quantities of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a significant product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Moreover, elevated ranges of HNE have been present in plasma and numerous organs underneath oxidative stress circumstances. The truth is, MDA is in lots of situations essentially the most ample particular person aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and lots of different biomolecules.

Bioquochem’s LPO assay equipment measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). Consequently, this response offers a diindolylalkane (chromophore) with most absorbance within the area of 580-620 nm.

In our assay an indol (Reagent A) reacts rapidly with MDA and HNE in acidic medium, yielding a chromophore (C) with a excessive molar extinction coefficient at its maximal absorption wavelength of 586 nm.

• Product overview

  Lipid Peroxidation (MDA) Assay Package (Colorimetric/Fluorometric) (ab118970) offers a handy software for delicate detection of malondialdehyde (MDA).

  Within the lipid peroxidation assay protocol, the MDA within the pattern reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct may be simply quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/properly colorimetrically and 0.1 nmol/properly fluorometrically.

  The MDA assay can also be refered to as a TBARS assay.

  Lipid peroxidation assay protocol abstract:
  – add TBA answer to samples and requirements, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
  – switch to wells of microplate
  – analyze with microplate reader
  For larger sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet earlier than evaluation.

  Chinese language protocol accessible. See protocols part beneath.

  For another MDA assay, with out the heating steps required within the TBARS assay, strive MDA assay ab233471.

• Notes

  Lipid peroxidation refers back to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (usually in cell membranes), leading to cell injury. Quantification of lipid peroxidation is important to evaluate oxidative stress. Lipid peroxidation varieties reactive aldehydes comparable to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are sometimes used as markers of lipid peroxidation, and to assay for oxidative injury / oxidative stress.

  Associated merchandise

  Evaluate the oxidative stress marker and assay information, or the complete metabolism assay information to study extra assays for metabolites, metabolic enzymes, mitochondrial perform, and oxidative stress, and in addition the best way to assay metabolic perform in reside cells utilizing your plate reader.

  Additionally see the favored 4-HNE Assay Package ab238538 instead marker of lipid peroxidation and oxidative stress.

   

  

  
  How different researchers have used Lipid Peroxidation Assay Package ab118970

  The MDA/TBARs assay equipment has been utilized in publications in quite a lot of pattern sorts, together with:
  – Human: serum¹, hippocampal major cell extracts², A375 cultured cell lysates³, plasma and platelet samples⁴
  – Mouse: neuronal cell lysates⁵, coronary heart tissue extract⁶, plasma⁷, cell extracts⁸
  – Rat: hippocampal tissue extracts⁹, cardiomyocyte extracts of cultured cells¹⁰, lung lysates¹¹
  – Pig: serum¹²

  ^{References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019}

• Platform

  Microplate reader

Lipid peroxidation Assay Kit
SH0312-3	Jiaxing Korain Biotech Ltd (BT Labs)	100T	EUR 880

Lipid peroxidation inhibitor 1
HY-U00319	MedChemExpress	1mg	EUR 1448.4

Lipid peroxidation inhibitor 1
T10166-10mg	TargetMol Chemicals	10mg	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
T10166-1g	TargetMol Chemicals	1g	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
T10166-1mg	TargetMol Chemicals	1mg	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
T10166-50mg	TargetMol Chemicals	50mg	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
T10166-5mg	TargetMol Chemicals	5mg	Ask for price
Description: Lipid peroxidation inhibitor 1

Lipid peroxidation inhibitor 1
MBS5765948-5mg	MyBiosource	5mg	EUR 915

Lipid peroxidation inhibitor 1
MBS5765948-5x5mg	MyBiosource	5x5mg	EUR 3970

Lipid Peroxidation (MDA) Assay Kit
abx096010-100Assays	Abbexa	100 Assays	EUR 453.6

Lipid Peroxidation MDA Assay Kit
EGQA0070	EnoGene	100T	EUR 85

Lipid Peroxidation (MDA) Assay Kit
K2167-500	ApexBio	500T	EUR 198.4
Description: Cell Biology|RNS detection

Lipid Peroxidation (MDA) Assay Kit
GWB-AXR340	GenWay Biotech	100 assays	Ask for price

Lipid Peroxidation (LPO) Assay Kit
abx096010-100l	Abbexa	100 µl	EUR 425

Lipid Peroxidation (LPO) Assay Kit
abx096010-1ml	Abbexa	1 ml	Ask for price

Lipid Peroxidation (LPO) Assay Kit
abx096010-200l	Abbexa	200 µl	Ask for price

Lipid Peroxidation (LPO)Assay Kit
BC015-50T	ELK Biotech	50T	EUR 200

Lipid Peroxidation (MDA) Assay kit
MBS9718963-48Tests	MyBiosource	48Tests	EUR 160

Lipid Peroxidation (MDA) Assay kit
MBS9718963-5x96Tests	MyBiosource	5x96Tests	EUR 745

Lipid Peroxidation (MDA) Assay kit
MBS9718963-96Tests	MyBiosource	96Tests	EUR 170

Lipid Peroxidation Colorimetric Assay Kit
K454-100	Biovision	each	EUR 652.8

Human Lipid Peroxidation (LPO) Elisa Kit
EK710559	AFG Bioscience LLC	96 Wells	EUR 0.1