Lipid Peroxidation (MDA + HNE) Assay Package

Lipid peroxidation is a well known instance of oxidative injury in cell membranes, lipoproteins, and different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl cholesterol can happen in several reactions. They are often triggered by i) free radical species comparable to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated discount of hydrogen peroxide or ii) non-radical species comparable to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are essential poisonous byproducts of lipid peroxidation. So, the measurement of the quantities of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a significant product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Moreover, elevated ranges of HNE have been present in plasma and numerous organs underneath oxidative stress circumstances. The truth is, MDA is in lots of situations essentially the most ample particular person aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and lots of different biomolecules.

Bioquochem’s LPO assay equipment measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). Consequently, this response offers a diindolylalkane (chromophore) with most absorbance within the area of 580-620 nm.

In our assay an indol (Reagent A) reacts rapidly with MDA and HNE in acidic medium, yielding a chromophore (C) with a excessive molar extinction coefficient at its maximal absorption wavelength of 586 nm.

• Product overview

  Lipid Peroxidation (MDA) Assay Package (Colorimetric/Fluorometric) (ab118970) offers a handy software for delicate detection of malondialdehyde (MDA).

  Within the lipid peroxidation assay protocol, the MDA within the pattern reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct may be simply quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/properly colorimetrically and 0.1 nmol/properly fluorometrically.

  The MDA assay can also be refered to as a TBARS assay.

  Lipid peroxidation assay protocol abstract:
  – add TBA answer to samples and requirements, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
  – switch to wells of microplate
  – analyze with microplate reader
  For larger sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet earlier than evaluation.

  Chinese language protocol accessible. See protocols part beneath.

  For another MDA assay, with out the heating steps required within the TBARS assay, strive MDA assay ab233471.

• Notes

  Lipid peroxidation refers back to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (usually in cell membranes), leading to cell injury. Quantification of lipid peroxidation is important to evaluate oxidative stress. Lipid peroxidation varieties reactive aldehydes comparable to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are sometimes used as markers of lipid peroxidation, and to assay for oxidative injury / oxidative stress.

  Associated merchandise

  Evaluate the oxidative stress marker and assay information, or the complete metabolism assay information to study extra assays for metabolites, metabolic enzymes, mitochondrial perform, and oxidative stress, and in addition the best way to assay metabolic perform in reside cells utilizing your plate reader.

  Additionally see the favored 4-HNE Assay Package ab238538 instead marker of lipid peroxidation and oxidative stress.

   

  

  
  How different researchers have used Lipid Peroxidation Assay Package ab118970

  The MDA/TBARs assay equipment has been utilized in publications in quite a lot of pattern sorts, together with:
  – Human: serum¹, hippocampal major cell extracts², A375 cultured cell lysates³, plasma and platelet samples⁴
  – Mouse: neuronal cell lysates⁵, coronary heart tissue extract⁶, plasma⁷, cell extracts⁸
  – Rat: hippocampal tissue extracts⁹, cardiomyocyte extracts of cultured cells¹⁰, lung lysates¹¹
  – Pig: serum¹²

  ^{References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019}

• Platform

  Microplate reader

Lipid Peroxidation Colorimetric Assay Kit
K454-100	Biovision	each	EUR 652.8

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K2167-100	ApexBio	100 assays	EUR 703.2

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K739-100	Biovision	each	EUR 698.4

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit
22906	AAT Bioquest	200 Tests	EUR 367.2

Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
15991	AAT Bioquest	200 tests	EUR 471.6

Myeloperoxidase (MPO) Peroxidation Activity Assay Kit
K747-100	Biovision	each	EUR 392.4

OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric)
STA-805	Cell Biolabs	192 assays	EUR 692.4
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample.

Lipid A Lipopolysaccharide (LPS Lipid A / Endotoxin) Antibody
abx415762-1ml	Abbexa	1 ml	EUR 727.2

Lipid A Antibody
abx023083-1ml	Abbexa	1 ml	EUR 886.8

Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free)
MET-5009-C	Cell Biolabs	50 preps	EUR 790.8
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis.

Ceraplex Lipid Concentrate(1000X)
C3316-010	GenDepot	100ml	EUR 144

Ceraplex Lipid Concentrate(1000X)
C3316-050	GenDepot	500ml	EUR 295.2

Lipid Droplet Isolation Kit
MET-5011	Cell Biolabs	50 preps	EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

Lipid Quantitation Kit (Colorimetric)
STA-613	Cell Biolabs	100 assays	EUR 637.2
Description: Cell Biolabs? Lipid Quantification Kit measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method, resulting in a simple colorimetric readout amenable to multi-well plate detection.

Lipid Quantification Kit (Fluorometric)
STA-617	Cell Biolabs	100 assays	EUR 637.2
Description: The Lipid Quantification Kit (Fluorometric) uses a fluorometric detection system to quantify neutral lipids in a convenient 96-well microplate reader. The kit includes sufficient reagents for 100 assays.

Anti-Lipid A antibody
STJ16100142	St John's Laboratory	1 mL	EUR 346.8

Monophosphoryl Lipid A (Synthetic)
VAdv-Ly0012-1mg	Creative Biolabs	1 mg	EUR 1033.2
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.

Monophosphoryl Lipid A (Synthetic)
VAdv-Ly0012-5mg	Creative Biolabs	5 mg	EUR 3013.2
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.

Synthetic Monophosphoryl Lipid A
VAdv-Ly0031	Creative Biolabs	1 mg	EUR 2557.2
Description: Synthetic monophosphoryl lipid A, a TLR4 agonist vaccine adjuvant.

MicroGram Lipid Assay Kit
LIP1000	ProFoldin	1000 assays	EUR 263.3
Description: This product includes 0.1 ml of 1000 x LIP 10 Dye. It is for measurement of 1000 samples using 96-well plates. Cuvettes may also be used for measurements.

LipidSpot 488 Lipid Droplet Stain
70065	Biotium	125uL	EUR 481.2
Description: Minimum order quantity: 1 unit of 125uL

LipidSpot 488 Lipid Droplet Stain
70065-T	Biotium	20uL	EUR 139.2
Description: Minimum order quantity: 1 unit of 20uL