FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue gentle inflicting molecules to change into excited and emit a visual yellow-green gentle. This emission ceases upon removing of the sunshine inflicting the excitation. Fluorochrome labeling offers speedy, correct localization of antigen-antibody interplay when one of many reactants is a part of a cell, tissue or different organic construction. FITC is a generally used marker for antibodies in immunofluorescent methods as a result of the conjugation of FITC to proteins is comparatively simple and usually doesn’t destroy the organic exercise of the labeled protein. FITC is extensively used as a hapten to label completely different proteins. Antibodies to FITC are used to establish FITC labeled proteins and as fashions to check the mechanism of antibody response to a properly outlined hapten.
Specificity
The antibody reacts with each free and conjugated fluorescein isothiocyanate (FITC).
Mouse monoclonal clone FL-D6 anti-FITC antibody will react with both free or conjugated FITC. The antibody doesn’t react with certain or free TRITC (tetramethylrhodamine isothiocyanate).
Immunogen
FITC-BSA conjugate
fd-rapid-golgistain-kit
Software
Mouse monoclonal clone FL-D6 anti-FITC antibody could also be used for the detection of FITC and as a common indicator reagent for bridging FITC with different immunochemical reagents. It could be utilized in ELISA, aggressive ELISA and immunofluorescent methods. A FITC Anti-FITC system has been used within the amplification of sign in immunofluorescent detection and as a method of separating certain from free tracer by affinity chromatography. The antibody may also be used to isolate cells which have an FITC-labeled ligand on their floor.
Disclaimer
Except in any other case acknowledged in our catalog or different firm documentation accompanying the product(s), our merchandise are meant for analysis use solely and usually are not for use for every other objective, which incorporates however just isn’t restricted to, unauthorized industrial makes use of, in vitro diagnostic makes use of, ex vivo or in vivo therapeutic makes use of or any kind of consumption or software to people or animals.
fd speedy golgistains
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Mouse Monoclonal Antibody
Elabscience’s expert technicians put together mouse monoclonal antibodies with first-class experimental tools and optimization operations, which might promise the last word consistency and efficiency.
Western Blot Detection Kits
Elabscience provides Western Blot Detection Kits which supplied for classical Western Blot experiments, together with all reagents wanted from protein extraction to consequence detection, which have benefits of straightforward operation, excessive detection sensitivity, low background and robust system stability. And Elabscience® offers detailed operation description {and professional} technical help. Moreover, it provides you comfort and assure to work out perfect Western Blot experiment outcomes. Click on to be taught extra in regards to the product.
Why sequence your monoclonal antibody (mAb)?
Patent Software – In the event you plan to acquire a patent in your mAb, antibody sequencing will likely be crucial with a view to distinguish your novel antibody from others.
Antibody Recombination – To find out the sequence of a mAb, the cDNA of your antibody could be remoted from a hybridoma cell line and its subcloning and expression in mammalian cells.
Antibody Humanization – Antibody sequence knowledge is important for any antibody engineering or humanization improvement.
Oak BioSciences provides quick and dependable cloning and sequencing companies for monoclonal antibodies. Our distinctive expertise permits us to sequence your antibodies from any antibody-producing cell line (hybridomas, peripheral blood lymphocytes, B-cells, spleen cells or bone marrow), and from a wide range of species (mouse, rat, hamster, rabbit, rooster, camelids, cartilaginous fishes, human and different primates). The service could be tailor-made to particular parameters, from variable areas or domains to full-length antibody sequencing.
The FDI FITC ANTI-RABIES MONOCLONAL GLOBULIN is used within the direct fluorescent antibody process for the in vitro detection of rabies in brains and submaxillary glands.The direct fluorescent antibody take a look at is the most effective single take a look at obtainable for the analysis of rabies. Research have proven that the fluorescent antibody technique for detection of rabies could also be extra delicate than the mouse inoculation take a look at;nevertheless, the outcomes of the 2 checks are 99-100% correlative.
