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Bioassay

QuantiChrom Urea Assay Kit

Utility

  • For quantitative dedication of urea and evaluation of drug outcomes on urea metabolism.

Key Choices

  • Delicate and proper. Use 5 μL samples. Linear detection differ 0.08 mg/dL (13 μM) to 100 mg/dL (17 mM) urea in 96-well plate assay.
  • Straightforward and high-throughput. The method contains addition of a single working reagent and incubation for 20 min. May very well be readily automated as a high-throughput assay for 1000’s of samples per day.
  • Improved reagent stability and adaptability. The optimized formulation has enormously enhanced reagent and signal stability. Cuvet or 96-well plate assay.
  • Low interference in natural samples. No pretreatments are needed. Assays may very well be straight carried out on raw natural samples i.e., throughout the presence of lipid and protein.

Approach

  •  OD520nm (Chemical)

Samples

  •  Serum, plasma, urine, milk, cell/tissue custom, bronchoalveolar lavage (BAL), meals, beverage and ambiance

Species

  •  All

Measurement

  •  100 assessments

Detection Limit

  •  0.08 mg/dL (13 μM)

Shelf Life

  •  12 months

Additional Particulars

  •  Urea is primarily produced throughout the liver and secreted by the kidneys. Urea is the primary end product of protein catabolism in animals. It is the most important vehicle for eradicating of toxic ammonia from the physique. Urea dedication may very well be very useful for the medical clinician to judge kidney function of victims. Principally, elevated urea ranges are associated to nephritis, renal ischemia, urinary tract obstruction, and positive extrarenal diseases, e.g., congestive coronary coronary heart failure, liver diseases and diabetes. Decreased ranges level out acute hepatic insufficiency or might final result from over-vigorous parenteral fluid treatment.
  • Straightforward, direct and automation-ready procedures for measuring urea focus or blood urea nitrogen BUN in natural samples have gotten customary in Evaluation and Drug Discovery. BioAssay Strategies urea assay gear is designed to measure urea straight in natural samples with none pretreatment. The improved Jung approach makes use of a chromogenic reagent that varieties a colored superior significantly with urea. The depth of the color, measured at 520nm, is straight proportional to the urea focus throughout the sample. The optimized formulation significantly reduces interference by substances throughout the raw samples.
urea-assay-kit
urea-assay-kit

For quantitative dedication of urea and evaluation of drug outcomes on urea metabolism.

  • Delicate and proper, use 5 μl samples
  • Linear detection differ 0.08 mg/dl (13 μM) to 100 mg/dl (17 mM) urea in 96-well plate assay
  • Straightforward and high-throughput
  • The method contains addition of a single working reagent and incubation for 20 min
  • May very well be readily automated as a high-throughput assay for 1000’s of samples per day
  • Approach: OD520 nm (Chemical)
  • Samples: Serum, plasma, urine, milk, cell/tissue custom, bronchoalveolar lavage (BAL), meals, beverage and ambiance

Improved reagent stability and adaptability. The optimized formulation has enormously enhanced reagent and signal stability. Low interference in natural samples. No pretreatments are needed. Assays may very well be straight carried out on raw natural samples i.e., throughout the presence of lipid and protein. Cuvet or 96-well plate assay. Urea is primarily produced throughout the liver and secreted by the kidneys. Urea is the primary end product of protein catabolism in animals.

It is the most important vehicle for eradicating of toxic ammonia from the physique. Urea dedication may very well be very useful for the medical clinician to judge kidney function of victims. Principally, elevated urea ranges are associated to nephritis, renal ischemia, urinary tract obstruction, and positive extrarenal diseases, e.g., congestive coronary coronary heart failure, liver diseases and diabetes.

Decreased ranges level out acute hepatic insufficiency or might final result from over-vigorous parenteral fluid treatment. Straightforward, direct and automation-ready procedures for measuring urea focus or blood urea nitrogen BUN in natural samples have gotten customary in Evaluation and Drug Discovery. BioAssay Strategies urea assay gear is designed to measure urea straight in natural samples with none pretreatment.

The improved Jung approach makes use of a chromogenic reagent that varieties a colored superior significantly with urea. The depth of the color, measured at 520 nm, is straight proportional to the urea focus throughout the sample. The optimized formulation significantly reduces interference by substances throughout the raw samples.

 

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