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Bioassay

QuantiChrom Urea Assay Kit

Utility

  • For quantitative dedication of urea and evaluation of drug outcomes on urea metabolism.

Key Choices

  • Delicate and proper. Use 5 μL samples. Linear detection differ 0.08 mg/dL (13 μM) to 100 mg/dL (17 mM) urea in 96-well plate assay.
  • Straightforward and high-throughput. The method contains addition of a single working reagent and incubation for 20 min. May very well be readily automated as a high-throughput assay for 1000’s of samples per day.
  • Improved reagent stability and adaptability. The optimized formulation has enormously enhanced reagent and signal stability. Cuvet or 96-well plate assay.
  • Low interference in natural samples. No pretreatments are needed. Assays may very well be straight carried out on raw natural samples i.e., throughout the presence of lipid and protein.

Approach

  •  OD520nm (Chemical)

Samples

  •  Serum, plasma, urine, milk, cell/tissue custom, bronchoalveolar lavage (BAL), meals, beverage and ambiance

Species

  •  All

Measurement

  •  100 assessments

Detection Limit

  •  0.08 mg/dL (13 μM)

Shelf Life

  •  12 months

Additional Particulars

  •  Urea is primarily produced throughout the liver and secreted by the kidneys. Urea is the primary end product of protein catabolism in animals. It is the most important vehicle for eradicating of toxic ammonia from the physique. Urea dedication may very well be very useful for the medical clinician to judge kidney function of victims. Principally, elevated urea ranges are associated to nephritis, renal ischemia, urinary tract obstruction, and positive extrarenal diseases, e.g., congestive coronary coronary heart failure, liver diseases and diabetes. Decreased ranges level out acute hepatic insufficiency or might final result from over-vigorous parenteral fluid treatment.
  • Straightforward, direct and automation-ready procedures for measuring urea focus or blood urea nitrogen BUN in natural samples have gotten customary in Evaluation and Drug Discovery. BioAssay Strategies urea assay gear is designed to measure urea straight in natural samples with none pretreatment. The improved Jung approach makes use of a chromogenic reagent that varieties a colored superior significantly with urea. The depth of the color, measured at 520nm, is straight proportional to the urea focus throughout the sample. The optimized formulation significantly reduces interference by substances throughout the raw samples.
urea-assay-kit
urea-assay-kit

For quantitative dedication of urea and evaluation of drug outcomes on urea metabolism.

  • Delicate and proper, use 5 μl samples
  • Linear detection differ 0.08 mg/dl (13 μM) to 100 mg/dl (17 mM) urea in 96-well plate assay
  • Straightforward and high-throughput
  • The method contains addition of a single working reagent and incubation for 20 min
  • May very well be readily automated as a high-throughput assay for 1000’s of samples per day
  • Approach: OD520 nm (Chemical)
  • Samples: Serum, plasma, urine, milk, cell/tissue custom, bronchoalveolar lavage (BAL), meals, beverage and ambiance

Improved reagent stability and adaptability. The optimized formulation has enormously enhanced reagent and signal stability. Low interference in natural samples. No pretreatments are needed. Assays may very well be straight carried out on raw natural samples i.e., throughout the presence of lipid and protein. Cuvet or 96-well plate assay. Urea is primarily produced throughout the liver and secreted by the kidneys. Urea is the primary end product of protein catabolism in animals.

It is the most important vehicle for eradicating of toxic ammonia from the physique. Urea dedication may very well be very useful for the medical clinician to judge kidney function of victims. Principally, elevated urea ranges are associated to nephritis, renal ischemia, urinary tract obstruction, and positive extrarenal diseases, e.g., congestive coronary coronary heart failure, liver diseases and diabetes.

Decreased ranges level out acute hepatic insufficiency or might final result from over-vigorous parenteral fluid treatment. Straightforward, direct and automation-ready procedures for measuring urea focus or blood urea nitrogen BUN in natural samples have gotten customary in Evaluation and Drug Discovery. BioAssay Strategies urea assay gear is designed to measure urea straight in natural samples with none pretreatment.

The improved Jung approach makes use of a chromogenic reagent that varieties a colored superior significantly with urea. The depth of the color, measured at 520 nm, is straight proportional to the urea focus throughout the sample. The optimized formulation significantly reduces interference by substances throughout the raw samples.

 

Urea Assay Kit

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Urea Assay Kit

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Description: Assay Kit for detection of Urea in the research laboratory

Urea Assay Kit

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Description: Cell Biolabs? Urea Assay Kit is based on the Berthelot reaction.  Urea is first degraded into ammonia and carbon dioxide, which further reacts with an alkaline developer to produce a blue-green colored product that can be measured with a standard spectrophotometric plate reader at an optical density between 580-630 nm.  Each kit provides sufficient reagents to perform up to 192 assays, including blanks, urea standards and unknown samples.

Urea Assay Kit

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Urea Colorimetric Assay Kit

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QuantiChrom™ Urea Assay Kit (100T)

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EUR 199
Description: For quantitative determination of urea and evaluation of drug effects on urea metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD520nm (Chemical). Samples: Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM).

EnzyChrom™ Urea Assay Kit III

EUR3-100 100
EUR 329
Description: For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 50 to 1000 µM urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD340nm. Samples: Urea in biological samples (e.g. plasma, serum, urine, bronchoalveolar lavage (BAL)) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 50 µM.

QuantiChrom™ Urea Assay Kit II

DUR2-100 100
EUR 189
Description: For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD557nm. Samples: Urea in biological samples (e.g. plasma, serum, urine) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 1 mg/mL (0.17 mM).

Urea Colorimetric Assay Kit II

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Blood Urea Nitrogen (BUN) Assay Kit

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Genorise® Urea Colorimetric Assay Kit

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Urease Activity Assay Kit

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QuantiChrom™ Urease Assay Kit

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Description: For quantitative determination of urease activity and evaluation/screen for urease inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD670nm. Samples: Biological, environment etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.003 U/L.

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