Utility
- For quantitative dedication of urea and evaluation of drug outcomes on urea metabolism.
Key Choices
- Delicate and proper. Use 5 μL samples. Linear detection differ 0.08 mg/dL (13 μM) to 100 mg/dL (17 mM) urea in 96-well plate assay.
- Straightforward and high-throughput. The method contains addition of a single working reagent and incubation for 20 min. May very well be readily automated as a high-throughput assay for 1000’s of samples per day.
- Improved reagent stability and adaptability. The optimized formulation has enormously enhanced reagent and signal stability. Cuvet or 96-well plate assay.
- Low interference in natural samples. No pretreatments are needed. Assays may very well be straight carried out on raw natural samples i.e., throughout the presence of lipid and protein.
Approach
- OD520nm (Chemical)
Samples
- Serum, plasma, urine, milk, cell/tissue custom, bronchoalveolar lavage (BAL), meals, beverage and ambiance
Species
- All
Measurement
- 100 assessments
Detection Limit
- 0.08 mg/dL (13 μM)
Shelf Life
- 12 months
Additional Particulars
- Urea is primarily produced throughout the liver and secreted by the kidneys. Urea is the primary end product of protein catabolism in animals. It is the most important vehicle for eradicating of toxic ammonia from the physique. Urea dedication may very well be very useful for the medical clinician to judge kidney function of victims. Principally, elevated urea ranges are associated to nephritis, renal ischemia, urinary tract obstruction, and positive extrarenal diseases, e.g., congestive coronary coronary heart failure, liver diseases and diabetes. Decreased ranges level out acute hepatic insufficiency or might final result from over-vigorous parenteral fluid treatment.
- Straightforward, direct and automation-ready procedures for measuring urea focus or blood urea nitrogen BUN in natural samples have gotten customary in Evaluation and Drug Discovery. BioAssay Strategies urea assay gear is designed to measure urea straight in natural samples with none pretreatment. The improved Jung approach makes use of a chromogenic reagent that varieties a colored superior significantly with urea. The depth of the color, measured at 520nm, is straight proportional to the urea focus throughout the sample. The optimized formulation significantly reduces interference by substances throughout the raw samples.
For quantitative dedication of urea and evaluation of drug outcomes on urea metabolism.
- Delicate and proper, use 5 μl samples
- Linear detection differ 0.08 mg/dl (13 μM) to 100 mg/dl (17 mM) urea in 96-well plate assay
- Straightforward and high-throughput
- The method contains addition of a single working reagent and incubation for 20 min
- May very well be readily automated as a high-throughput assay for 1000’s of samples per day
- Approach: OD520 nm (Chemical)
- Samples: Serum, plasma, urine, milk, cell/tissue custom, bronchoalveolar lavage (BAL), meals, beverage and ambiance
Improved reagent stability and adaptability. The optimized formulation has enormously enhanced reagent and signal stability. Low interference in natural samples. No pretreatments are needed. Assays may very well be straight carried out on raw natural samples i.e., throughout the presence of lipid and protein. Cuvet or 96-well plate assay. Urea is primarily produced throughout the liver and secreted by the kidneys. Urea is the primary end product of protein catabolism in animals.
It is the most important vehicle for eradicating of toxic ammonia from the physique. Urea dedication may very well be very useful for the medical clinician to judge kidney function of victims. Principally, elevated urea ranges are associated to nephritis, renal ischemia, urinary tract obstruction, and positive extrarenal diseases, e.g., congestive coronary coronary heart failure, liver diseases and diabetes.
Decreased ranges level out acute hepatic insufficiency or might final result from over-vigorous parenteral fluid treatment. Straightforward, direct and automation-ready procedures for measuring urea focus or blood urea nitrogen BUN in natural samples have gotten customary in Evaluation and Drug Discovery. BioAssay Strategies urea assay gear is designed to measure urea straight in natural samples with none pretreatment.
The improved Jung approach makes use of a chromogenic reagent that varieties a colored superior significantly with urea. The depth of the color, measured at 520 nm, is straight proportional to the urea focus throughout the sample. The optimized formulation significantly reduces interference by substances throughout the raw samples.
 Urea Assay Kit |
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| abx098464-Hitachi7060R190ml2R245ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 45ml×1 | EUR 222 |
Urea Assay Kit |
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| abx098464-Toshiba120R140ml4R240ml1 | Abbexa | Toshiba 120; R1: 40ml×4 R2: 40ml×1 | EUR 222 |
Urea Assay Kit |
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| Z5030016 | Biochain | 500 assays | EUR 574 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Urea Assay Kit |
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| STA-382 | Cell Biolabs | 192 assays | EUR 635 |
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Description: Cell Biolabs? Urea Assay Kit is based on the Berthelot reaction. Urea is first degraded into ammonia and carbon dioxide, which further reacts with an alkaline developer to produce a blue-green colored product that can be measured with a standard spectrophotometric plate reader at an optical density between 580-630 nm. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, urea standards and unknown samples. |
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 Urea Colorimetric Assay Kit |
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| K375-100 | Biovision | EUR 539 | |
 QuantiChrom Urea Assay Kit II |
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| DUR2-100 | BioAssay Systems | 100 | EUR 189 |
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Description: quantitative enzymatic determination of urea |
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) QuantiChrom Urea Assay Kit (100T) |
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| DIUR-100 | BioAssay Systems | 100 | EUR 200 |
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Description: Quantitative determination of urea by chemical colorimetric (520nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM). Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C. |
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 EnzyChrom Urea Assay Kit III |
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| EUR3-100 | BioAssay Systems | 100 | EUR 297 |
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Description: quantitative enzymatic determination of urea |
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 Urea Colorimetric Assay Kit II |
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| K376-100 | Biovision | EUR 539 | |
 Assay Kit) Blood Urea Nitrogen (BUN) Assay Kit |
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| abx090684-100tests | Abbexa | 100 tests | EUR 237 |
 Urea |
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| abx186596-5g | Abbexa | 5 g | EUR 189 |
Urea |
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| 20-abx082354 | Abbexa |
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Urea |
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| 20-abx082586 | Abbexa |
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Urea |
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| B7781-1000000 | ApexBio | 1 kg | EUR 163 |
Urea |
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| GE3329-1KG | Glentham Life Sciences | 1 kg | EUR 47 |
Urea |
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| GE3329-250G | Glentham Life Sciences | 250 g | EUR 38 |
Urea |
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| GE3329-500G | Glentham Life Sciences | 500 g | EUR 42 |
Urea |
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| GE3329-5KG | Glentham Life Sciences | 5 kg | EUR 86 |
Urea |
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| HY-Y0271 | MedChemExpress | 100mg | EUR 108 |
Urea |
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| UB0148 | Bio Basic | 500g | EUR 58.27 |
 ureA Antibody |
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| 1-CSB-PA14347A0Rb | Cusabio |
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Description: A polyclonal antibody against ureA. Recognizes ureA from Helicobacter pylori. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000 |
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 Dimethylol urea |
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| 20-abx180054 | Abbexa |
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 Imidazolidinyl urea |
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| HY-B1158 | MedChemExpress | 10mM/1mL | EUR 113 |
 250G Urea |
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| NAT1076 | National Diagnostics | 250G | EUR 82 |
