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ATpase assay

ATpase assay

BioVision affords complete in vitro bioassays which can be utilized by hundreds of worldwide scientists in quite a few illness associated areas like most cancers, diabetes, weight problems, heart problems, liver illness, kidney illness, Alzheimer’s illness and infectious ailments, and many others., in addition to different analysis areas comparable to metabolism, neuroscience, epigenetics, oxidative stress, stem cells, protein extraction and pattern preparation, and many others. Our high-quality bioassays provide specificity, sensitivity, reproducibility and but reasonably priced. Our mission is to ship revolutionary bioassays primarily based on innovative analysis to satisfy your scientific wants. BioVision’s bioassay kits can be utilized with a number of the hottest and customary analysis instrumentation (i.e. microplate reader, circulation cytometer, and microscopes).

ATPase Activity Assay Kit
ATPase Exercise Assay Equipment

Inorganic ions play quite a few physiological roles in nature. Cations and anions take part in neurological transmission, muscle contraction, intra-cellular water transport, acid-base steadiness in blood, bone formation, power switch and storage. Moreover, they operate as cofactors or might be discovered as moieties inside
different necessary biomolecules. BioVision has an incredible portfolio of assays measuring inorganic ions together with assays for sodium, calcium, phosphate and lots of others.

Key Options:

Non-radioactive, homogeneous assays
• Particular assays
• Handy: minimal pattern preparation; quick protocols (1-2 hours )
• Price efficient: 100 assays;
• Excessive Throughput Screening suitable
• Validated: utilizing mammalian tissues, cells, organic fluids

BioVision’s intensive microplate-based assay portfolio affords quite a few merchandise that can be utilized for the detection/quantification of enzymatic exercise, quantification of particular chemical analytes in organic samples and for
high-throughput inhibitor screening. Our merchandise make the most of mixtures of proprietary enzymes and chemical probes, fastidiously engineered and characterised for the detection of related targets. Our kits have been examined and validated utilizing actual organic samples (comparable to blood, serum, plasma, urine, saliva, cell and tissue lysates and foodstuffs), guaranteeing that analytes are detectable in real-world organic matrices.

Pattern utility is maybe a very powerful issue when selecting the right product on your analysis wants. This may decide the mandatory assay sensitivity and due to this fact the popular detection methodology. Our hottest merchandise make the most of secure chromophores and fluorophores that present sturdy alerts over time. PicoProbe, our proprietary fluorophore, is without doubt one of the most delicate probes out there. It may possibly detect as little as 20 pmoles of analyte per analyzed pattern effectively.

ATPase Activity Assay Kit (Colorimetric)
ATPase Exercise Assay Equipment (Colorimetric)

Metabolism performs a key position in numerous areas comparable to physiology, cell biology, and drugs. Analysis in metabolism is dramatically elevated by the belief that many widespread human ailments comparable to most cancers, diabetes, weight problems, hypoglycemia, hypolipidemia, phenylketonuria, neurodegeneration, and many others. are resulting from perturbations in metabolic pathways. Understanding the metabolic variations and potential outliers is thus an important side of disease-oriented analysis. Then again, reactive oxygen species (ROS), produced endogenously on account of regular mobile metabolic capabilities or from exterior sources, poses a big risk to cells since publicity to ROS can lead to severedamage to DNA, protein, and lipids. Figuring out particular types of oxidative harm is usually the focus of curiosity  in several types of cancers and numerous neurological ailments. Antioxidant defenses to attenuate fluctuations in ROS are crucial to cell survival and dictate approaches to illness therapies to scale back danger of most cancers or age-related purposeful deterioration.

 

Nonetheless, organic processes are higher characterised by learning mobile interplay resulting from exogenous elements. Preclinical and Medical Analysis depend on assays that examine completely different points of mobile conduct (development via the cell cycle, migration, invasion) and measure the content material of key constructing blocks within the cell are generally used to observe the well being of cells in tradition and their response to numerous stimuli (migration inducers, cell cycle arrest medicine, ldl cholesterol transport inhibitors and many others.).
BioVision is proud to supply the broadest Metabolism Assay portfolio starting from the detection of enzymatic exercise, metabolites comparable to carbohydrates, lipids, amino acids, inorganic ions, serum parts, coenzymes & cofactors to oxidative stress, glycolysis pathway, and middleman metabolism. Additionally, we provide a good collection of assay kits, antioxidants, inhibitors/activators of key ROS gamers and a increasing collection of assays to check some elementary processes governing mobile biology.

QuantiChrom ATPase/GTPase Assay Kit

DATG-200 200
EUR 486
Description: Quantitative determination of ATPase or GTPase activity by colorimetric (620nm) method. High-throµghput screening for ATPase/GTPase inhibitors. Procedure: 30 min. Kit size: 200 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

ATPase Activity Assay Kit (Colorimetric)

K417-100
EUR 516

E. coli DNA Helicase ATPase assay Kit Plus-100

DNAB100KE 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x E. coli DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-100

DNAB100KH 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x H. influenzae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-100

DNAB100KN 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. pneumoniae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-100

DNAB100KP 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x P. aeruginosa DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-100

DNAB100KS 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. aureus DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

E. coli DNA Helicase ATPase assay Kit Plus-500

DNAB500KE 500 assays
EUR 1396.74
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x E. coli DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-500

DNAB500KH 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x H. influenzae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-500

DNAB500KN 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. pneumoniae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-500

DNAB500KP 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x P. aeruginosa DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-500

DNAB500KS 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. aureus DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

E. coli gyrase ATPase assay Kit Plus (enzyme included)

T2A-100KE 100 assays
EUR 444.91
Description: This product includes the reaction buffer, DNA, E. coli gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

P. aeruginosa Gyrase ATPase assay Kit Plus (enzyme included)

T2A-100KP 100 assays
EUR 444.91
Description: This product includes the reaction buffer, DNA, P. aeruginosa gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

S. aureus gyrase ATPase assay Kit Plus (enzyme included)

T2A-100KS 100 assays
EUR 423.72
Description: This product includes the reaction buffer, DNA, S. aureus gyrase, ATP and the phosphate detection reagent for 100 assays of DNA supercoiling reactions in a 384-well assay format.

E. coli Topo IV ATPase Assay Kit Plus (enzyme included)

T4A-100KE 100 assays
EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x E. coli Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of E. coli Topo IV ATPase activity in a 384-well assay format.

S. pneumoniae Topo IV ATPase Assay Kit Plus (enzyme included)

T4A-100KN 100 assays
EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x S. pneumoniae Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of S. pneumoniae Topo IV ATPase activity in a 384-well assay format.

P. aeruginosa Topoisomerase IV ATPase Assay Kit Plus (enzyme included)

T4A-100KP 100 assays
EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x P. aeruginosa Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of P. aeruginosa Topo IV ATPase activity in a 384-well assay format.

S. aureus Topoisomerase IV ATPase Assay Kit Plus (enzyme included)

T4A-100KS 100 assays
EUR 444.91
Description: This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x S. aureus Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of S. aureus Topo IV ATPase activity in a 384-well assay format.

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